Hi thegoodthebadandme – yes there are certainly techniques that are better than others when obtaining the cells needed. Hopefully one of the other lab researchers can give you some more details!
Hi thegoodthebadandme,
There are lots of different ways to extract stem cells, I’m going to tell you about embryonic stem cells as this is what I do, but adult stem cells are extracted differently, and this depends on what kind of adult stem cells they are.
Back to embryonic stem cells, there are 2 main ways that I use to extract them. The first is just by cutting out the inner cells of the embryo using a microscope and very tiny sharp needles. it’s a kind of cutting and flicking movement and you gently tease the cells apart. Its very tricky as the embryo jumps about a lot and the cells are VERY sticky and stick to the needles!
The second method I use uses antibodies to dissolve the outer cells, leaving the inner cells intact, this is called immunosurgery. I think this is harder as it is easy to leave the cells in the antibodies too long, you can damage the inner cells so you need to be really careful! I sometimes use a combination of both methods.
Other scientists use other methods involving lasers and fancy cutting tools! I’ve never seen these in action tho!
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